![]() Highlighted region in blue with a Red vector line is the 72bp insert manually added to the assembly to test for more accurate read mapping. Pfizer monovalent vaccine annotated with SnapGene. Both monovalent and bivalent Pfizer vaccines contain 2 copies of the 72bp Enhancer in the SV40 promoter.įigure 2. This implies that Megahit is struggling to assemble a 72bp tandem repeat with 105bp insert sizes. Once we inserted an additional copy of the 72bp element into the Megahit constructed reference (shown in red in Figure 2), the coverage pile up disappeared. ![]() This mis-assembly was also reproduced from sequence data derived from an independent group in Europe (Voor Waarheid) who has also sequenced BNT162b2 (personal communication and in prep). The initial assembly also showed signs of a read coverage pile up across the 72bp enhancer in the SV40 promoter described in figure 5A and figure 5B of this preprint. This vector is nearly identical to the bivalent vectors recently assembled. A 7,754 base pair spike encoded plasmid was assembled with a truncated Poly A tract and a previously described mis-assembly of the SV40 promoter region. Using methods described earlier, we sequenced the Pfizer monovalent BNT162b2 vaccines and assembled these reads with Megahit. ![]() ![]() Depiction of the 72bp tandem repeat in the SV40 promoter of BNT162b2 vaccine. ![]()
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